A LABORATORY PROTOCOL ON THE BIOLOGY  EXPERIMENT CARRIED OUT BY BELGIAN STUDENTS OF ZAVO (BELGIUM)

The influence of acid rain on vascular and non-vascular plants

necessary equipment: 

  • 2 measuring cups
  • 1 vascular plant (fern, seed plant or similar)
  • 1 non-vascular plant (moss, liverworts, hornworts or similar)
  • 2 shallow bowls or pots to hold the plants
  • ph test strips
  • ½ liter of fresh water
  • small bottle of vinegar
  • safety goggles
  • latex gloves
  • (if possible) lab coats

safety measures:

  • wear goggles at all times
  • wear latex gloves when handling materials

procedure:

  • measure the ph-value of both plants using the test strips and note the value in the first column
  • take a picture of both plants
  • divide the fresh water equally into both measuring cups
  • mix a small amount of vinegar in both measuring cups, make sure to use the exact same amount both times
  • measure the ph-value in both cups and note the value in the second column
  • water each plant with the same amount of the prepared ‘acid rain’
  • wait 20 minutes
  • measure the ph-value of both plants using the test strips and note the value in the third column
  • take a new picture of both plants from the same angle as the first time
  • compare the first and third column and compare the first and second picture of both plants
  • form conclusion